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Intravital Imaging of Dynamic Bone and Immune Systems

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Cover of 'Intravital Imaging of Dynamic Bone and Immune Systems'

Table of Contents

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    Book Overview
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    Chapter 1 Bone Imaging: Osteoclast and Osteoblast Dynamics
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    Chapter 2 Intravital Imaging of Mouse Bone Marrow: Hemodynamics and Vascular Permeability
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    Chapter 3 Bone Imaging: Platelet Formation Dynamics
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    Chapter 4 Live Imaging of Interstitial T Cell Migration Using Lymph Node Slices
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    Chapter 5 Two-Photon Imaging of T-Cell Motility in Lymph Nodes: In Vivo and Ex Vivo Approaches
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    Chapter 6 Imaging the Lymph Node Stroma
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    Chapter 7 Intravital Imaging of B Cell Responses in Lymph Nodes
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    Chapter 8 Live Imaging of the Skin Immune Responses: Visualization of the Contact Hypersensitivity Response
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    Chapter 9 Imaging of Inflammatory Responses in the Mouse Ear Skin
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    Chapter 10 In Vivo Imaging of Immune Cells in Peyer’s Patches
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    Chapter 11 Intravital Imaging of T Cells Within the Spinal Cord
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    Chapter 12 Kidney Imaging: Intravital Microscopy
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    Chapter 13 Intravital Imaging of Liver Cell Dynamics
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    Chapter 14 Intravital Imaging of the Heart at the Cellular Level Using Two-Photon Microscopy
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    Chapter 15 Imaging Window Device for Subcutaneous Implantation Tumor
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    Chapter 16 New Tools for Imaging of Immune Systems: Visualization of Cell Cycle, Cell Death, and Cell Movement by Using the Mice Lines Expressing Fucci, SCAT3.1, and Kaede and KikGR
Attention for Chapter 3: Bone Imaging: Platelet Formation Dynamics
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Chapter title
Bone Imaging: Platelet Formation Dynamics
Chapter number 3
Book title
Intravital Imaging of Dynamic Bone and Immune Systems
Published in
Methods in molecular biology, February 2018
DOI 10.1007/978-1-4939-7762-8_3
Pubmed ID
Book ISBNs
978-1-4939-7761-1, 978-1-4939-7762-8
Authors

Asuka Sakata, Satoshi Nishimura, Sakata, Asuka, Nishimura, Satoshi

Abstract

Bone imaging using multiphoton microscopy enables dynamic observation of platelet formation in living animals. Two-photon excitation microscopy is superior to confocal microscopy as it enables deeper tissue penetration, efficient light detection, and reduced phototoxicity. Using this microscopy approach, thrombopoiesis by megakaryocytes is clearly visualized in the skull at significant depth from the surface. Here we describe our microscopy setup and dye recipe for visualization of bone marrow in the mouse skull.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 3 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 3 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 1 33%
Student > Master 1 33%
Unknown 1 33%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 2 67%
Unknown 1 33%