Chapter title |
Stable Delivery of CCR5-Directed shRNA into Human Primary Peripheral Blood Mononuclear Cells and Hematopoietic Stem/Progenitor Cells via a Lentiviral Vector.
|
---|---|
Chapter number | 19 |
Book title |
SiRNA Delivery Methods
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3112-5_19 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3111-8, 978-1-4939-3112-5
|
Authors |
Shimizu, Saki, Yadav, Swati Seth, An, Dong Sung, Saki Shimizu, Swati Seth Yadav, Dong Sung An |
Abstract |
RNAi is a powerful tool to achieve suppression of a specific gene expression and therefore it has tremendous potential for gene therapy applications. A number of vector systems have been developed to express short-hairpin RNAs (shRNAs) to produce siRNAs within mammalian T-cells, primary hematopoietic stem/progenitor cells (HSPC), human peripheral blood mononuclear cells, and in animal model systems. Among these, vectors based on lentivirus backbones have significantly transformed our ability to transfer shRNAs into nondividing cells, such as HSPC, resulting in high transduction efficiencies. However, delivery and long-term expression of shRNAs should be carefully optimized for efficient knock down of target gene without causing cytotoxicity in mammalian cells. Here, we describe our protocols for the development of shRNA against a major HIV co-receptor/chemokine receptor CCR5 and the use of lentiviral vectors for stable shRNA delivery and expression in primary human PBMC and HSPC. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Science communicators (journalists, bloggers, editors) | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 17 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 4 | 24% |
Researcher | 4 | 24% |
Student > Master | 4 | 24% |
Student > Bachelor | 2 | 12% |
Student > Doctoral Student | 1 | 6% |
Other | 2 | 12% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 5 | 29% |
Biochemistry, Genetics and Molecular Biology | 4 | 24% |
Medicine and Dentistry | 3 | 18% |
Nursing and Health Professions | 1 | 6% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 6% |
Other | 2 | 12% |
Unknown | 1 | 6% |