Chapter title |
Hepatic Delivery of Artificial Micro RNAs Using Helper-Dependent Adenoviral Vectors.
|
---|---|
Chapter number | 20 |
Book title |
SiRNA Delivery Methods
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3112-5_20 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3111-8, 978-1-4939-3112-5
|
Authors |
Crowther, Carol, Mowa, Betty, Arbuthnot, Patrick, Carol Crowther, Betty Mowa, Patrick Arbuthnot |
Abstract |
The potential of RNA interference (RNAi)-based gene therapy has been demonstrated in many studies. However, clinical application of this technology has been hampered by a paucity of efficient and safe methods of delivering the RNAi activators. Prolonged transgene expression and improved safety of helper-dependent adenoviral vectors (HD AdVs) makes them well suited to delivery of engineered artificial intermediates of the RNAi pathway. Also, AdVs' natural hepatotropism makes them potentially useful for liver-targeted gene delivery. HD AdVs may be used for efficient delivery of cassettes encoding short hairpin RNAs and artificial primary microRNAs to the mouse liver. Methods for the characterization of HD AdV-mediated delivery of hepatitis B virus-targeting RNAi activators are described here. |
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