| Chapter title |
SiRNA In Vivo-Targeted Delivery to Murine Dendritic Cells by Oral Administration of Recombinant Yeast.
|
|---|---|
| Chapter number | 14 |
| Book title |
SiRNA Delivery Methods
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3112-5_14 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3111-8, 978-1-4939-3112-5
|
| Authors |
Xu, Kun, Liu, Zhongtian, Zhang, Long, Zhang, Tingting, Zhang, Zhiying, Kun Xu, Zhongtian Liu, Long Zhang, Tingting Zhang, Zhiying Zhang |
| Abstract |
SiRNA therapeutics promise a future where any target in the transcriptome could be potentially addressed. However, the delivery of SiRNAs and targeting of particular cell types or organs are major challenges. A novel, efficient, and safe delivery system for promising the introduction of SiRNAs into particular cell types within living organisms is of great significance. Our previous studies have proved that recombinant protein (MSTN) and exogenous gene (EGFP) as vaccines, and furthermore functional CD40 shRNA expression can be delivered into dendritic cells (DCs) in mouse by oral administration of recombinant yeast (Saccharomyces cerevisiae). Here, we describe the details of the promising and innovative approach based on oral administration of recombinant yeast that allows in vivo-targeted delivery of functional SiRNA to murine intestinal DCs. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 33% |
| Unknown | 2 | 67% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 67% |
| Science communicators (journalists, bloggers, editors) | 1 | 33% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 9 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 2 | 22% |
| Lecturer | 1 | 11% |
| Professor | 1 | 11% |
| Student > Master | 1 | 11% |
| Researcher | 1 | 11% |
| Other | 0 | 0% |
| Unknown | 3 | 33% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 3 | 33% |
| Biochemistry, Genetics and Molecular Biology | 1 | 11% |
| Chemistry | 1 | 11% |
| Engineering | 1 | 11% |
| Unknown | 3 | 33% |