Nanomanufacture of Free-Standing, Porous, Janus-Type Films of Polymer–Plant Virus Nanoparticle Arrays
Virus-Derived Nanoparticles for Advanced Technologies
Methods in molecular biology, January 2018
Brylee David B. Tiu, Rigoberto C. Advincula, Nicole F. Steinmetz, Tiu, Brylee David B., Advincula, Rigoberto C., Steinmetz, Nicole F.
We present a facile method for preparing hierarchical assemblies of cowpea mosaic virus (CPMV) nanoparticles adsorbed onto patterned polypyrrole copolymer arrays, which can be released as a freely standing and microporous polymer-protein membrane with a Janus-type structure. The patterning protocol is based on colloidal sphere lithography wherein a sacrificial honeycomb pattern composed of colloidal polystyrene (PS) microspheres is assembled on an electrode. A thin layer of polypyrrole film is electropolymerized within the interstices of the template and monitored using an electrochemical quartz crystal microbalance with dissipation (EC-QCM-D) and microscopy. Dissolving the PS template reveals an inverse opaline pattern capable of electrostatically capturing the CPMV particles. Through an electrochemical trigger, the polypyrrole-CPMV delaminates from the surface producing a self-sustaining polymer-protein membrane that can potentially be used for sensing and nanocargo applications.
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