Chapter title |
Production and Application of Insect Virus-Based VLPs
|
---|---|
Chapter number | 8 |
Book title |
Virus-Derived Nanoparticles for Advanced Technologies
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7808-3_8 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7806-9, 978-1-4939-7808-3
|
Authors |
Radhika Gopal, Anette Schneemann, Gopal, Radhika, Schneemann, Anette |
Abstract |
Virus-like particles (VLPs) are self-assembling platforms composed of viral structural proteins. They are used for a variety of purposes, ranging from the study of virus assembly to vaccine development. VLPs can be produced in plants, bacteria, yeast, and insect and mammalian cells. The baculovirus expression system is one of the most commonly used systems for production of VLPs in eukaryotic cells. This chapter provides a brief overview of the main strategies used to generate recombinant baculoviruses and the applications of insect virus-derived VLPs in basic and applied research. It then describes detailed protocols for generation of recombinant baculoviruses, screening for their expression of VLPs in insect cells, and VLP purification. |
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