| Chapter title |
Image Analysis of DNA Fiber and Nucleus in Plants
|
|---|---|
| Chapter number | 13 |
| Book title |
Chromosome and Genomic Engineering in Plants
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-4931-1_13 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-4929-8, 978-1-4939-4931-1
|
| Authors |
Nobuko Ohmido, Toshiyuki Wako, Seiji Kato, Kiichi Fukui |
| Abstract |
Advances in cytology have led to the application of a wide range of visualization methods in plant genome studies. Image analysis methods are indispensable tools where morphology, density, and color play important roles in the biological systems. Visualization and image analysis methods are useful techniques in the analyses of the detailed structure and function of extended DNA fibers (EDFs) and interphase nuclei. The EDF is the highest in the spatial resolving power to reveal genome structure and it can be used for physical mapping, especially for closely located genes and tandemly repeated sequences. One the other hand, analyzing nuclear DNA and proteins would reveal nuclear structure and functions. In this chapter, we describe the image analysis protocol for quantitatively analyzing different types of plant genome, EDFs and interphase nuclei. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 3 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Lecturer > Senior Lecturer | 1 | 33% |
| Professor | 1 | 33% |
| Professor > Associate Professor | 1 | 33% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 1 | 33% |
| Agricultural and Biological Sciences | 1 | 33% |
| Unknown | 1 | 33% |