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Mendeley readers
| Chapter title |
Method for Biolistic Site-Specific Integration in Plants Catalyzed by Bxb1 Integrase
|
|---|---|
| Chapter number | 2 |
| Book title |
Chromosome and Genomic Engineering in Plants
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-4931-1_2 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-4929-8, 978-1-4939-4931-1
|
| Authors |
Ruyu Li, Zhiguo Han, Lili Hou, Gurminder Kaur, Qian Yin, David W. Ow |
| Abstract |
Crop improvement is a never ending process. With a transgenesis approach, it is not inconceivable to envision a continuous addition of new transgenes to existing cultivars. Previously, we described a recombinase-directed gene stacking method in tobacco (Hou et al., Mol Plant 7:1756-1765, 2014). Being able to stack DNA to a previous location ensures that the number of genetic loci does not increase with each new round of transgene addition. Whereas the previous demonstration was conducted through polyethylene glycol to mediate uptake of DNA into tobacco protoplasts, we now describe protocols for using biolistic transformation to stack DNA in tobacco and rice. |
Mendeley readers
The data shown below were compiled from readership statistics for 3 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 3 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 2 | 67% |
| Unknown | 1 | 33% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 2 | 67% |
| Unknown | 1 | 33% |