| Chapter title |
Protocol for In Vitro Stacked Molecules Compatible with In Vivo Recombinase-Mediated Gene Stacking
|
|---|---|
| Chapter number | 3 |
| Book title |
Chromosome and Genomic Engineering in Plants
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-4931-1_3 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-4929-8, 978-1-4939-4931-1
|
| Authors |
Weiqiang Chen, David W. Ow |
| Abstract |
Previously, we described a method for a recombinase-directed stacking of new DNA to an existing transgenic locus. Here, we describe how we can similarly stack DNA molecules in vitro and that the in vitro derived gene stack can be incorporated into an Agrobacterium transformation vector by in vitro recombination. After transfer to the chromosome by Agroinfection, the transgenic locus harbors a new target site that can be used for the subsequent in vivo stacking of new DNA. Alternatively, the in vitro derived gene stack has the potential to be integrated directly into the plant genome in vivo at a preexisting chromosomal target. Being able to stack DNA in vitro as well as in vivo, and with compatibility between the two systems, brings new flexibility for using the recombinase-mediated approach for transgene stacking. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 4 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 2 | 50% |
| Professor > Associate Professor | 1 | 25% |
| Student > Ph. D. Student | 1 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 2 | 50% |
| Immunology and Microbiology | 1 | 25% |
| Unknown | 1 | 25% |