Chapter title |
Production of Engineered Minichromosome Vectors via the Introduction of Telomere Sequences
|
---|---|
Chapter number | 1 |
Book title |
Chromosome and Genomic Engineering in Plants
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-4931-1_1 |
Pubmed ID | |
Book ISBNs |
978-1-4939-4929-8, 978-1-4939-4931-1
|
Authors |
Nathaniel Graham, Nathan Swyers, Jon Cody, Morgan McCaw, Changzeng Zhao, James A. Birchler |
Abstract |
Artificial minichromosomes are non-integrating vectors capable of stably maintaining transgenes outside of the main chromosome set. The production of minichromosomes relies on telomere-mediated chromosomal truncation, which involves introducing transgenes and telomere sequences concurrently to the cell to truncate an endogenous chromosomal target. Two methods can be utilized; either the telomere sequences can be incorporated into a binary vector for transformation with Agrobacterium tumefaciens, or the telomere sequences can be co-introduced with transgenes during particle bombardment. In this protocol, the methods required to isolate and introduce telomere sequences are presented. Following the methods presented, standard transformation procedures can be followed to produce minichromosome containing plants. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 10 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 3 | 30% |
Student > Master | 2 | 20% |
Student > Bachelor | 1 | 10% |
Researcher | 1 | 10% |
Student > Postgraduate | 1 | 10% |
Other | 0 | 0% |
Unknown | 2 | 20% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 3 | 30% |
Biochemistry, Genetics and Molecular Biology | 2 | 20% |
Environmental Science | 1 | 10% |
Medicine and Dentistry | 1 | 10% |
Unknown | 3 | 30% |