Chapter title |
Flow Cytometry Protocols for Assessment of Platelet Function in Whole Blood
|
---|---|
Chapter number | 28 |
Book title |
Hemostasis and Thrombosis
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-7196-1_28 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7194-7, 978-1-4939-7196-1
|
Authors |
Leonardo Pasalic, Gabrielle J. Pennings, David Connor, Heather Campbell, Leonard Kritharides, Vivien M. Chen, Pasalic, Leonardo, Pennings, Gabrielle J., Connor, David, Campbell, Heather, Kritharides, Leonard, Chen, Vivien M. |
Abstract |
Flow cytometry is a powerful tool for rapid evaluation of multiple functional properties of large numbers of platelets in whole blood. In the following chapter, we provide a number of flow cytometry-based protocols broadly aimed at (1) assessment of constitutively expressed platelet membrane receptors to diagnose inherited platelet bleeding disorders and (2) investigation of basal and agonist-induced platelet functional responses including generation of platelet-leukocyte aggregates, alpha and dense granule release, calcium flux, and phosphatidylserine exposure. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 50 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 10 | 20% |
Student > Ph. D. Student | 8 | 16% |
Student > Bachelor | 8 | 16% |
Student > Master | 6 | 12% |
Lecturer | 2 | 4% |
Other | 5 | 10% |
Unknown | 11 | 22% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 12 | 24% |
Biochemistry, Genetics and Molecular Biology | 10 | 20% |
Agricultural and Biological Sciences | 4 | 8% |
Immunology and Microbiology | 2 | 4% |
Engineering | 2 | 4% |
Other | 7 | 14% |
Unknown | 13 | 26% |