Chapter title |
Generation and Characterization of Mouse Regulatory Macrophages
|
---|---|
Chapter number | 6 |
Book title |
Suppression and Regulation of Immune Responses
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3139-2_6 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3138-5, 978-1-4939-3139-2
|
Authors |
Laura Carretero-Iglesia, Marcelo Hill, Maria Cristina Cuturi, Carretero-Iglesia, Laura, Hill, Marcelo, Cuturi, Maria Cristina |
Abstract |
In the last years, cell therapy has become a promising approach to therapeutically manipulate immune responses in autoimmunity, cancer, and transplantation. Several types of lymphoid and myeloid cells origin have been generated in vitro and tested in animal models. Their efficacy to decrease pharmacological treatment has successfully been established. Macrophages play an important role in physiological and pathological processes. They represent an interesting cell population due to their high plasticity in vivo and in vitro. Here, we describe a protocol to differentiate murine regulatory macrophages in vitro from bone marrow precursors. We also describe several methods to assess macrophage classical functions, as their bacterial killing capacity and antigen endocytosis and degradation. Importantly, regulatory macrophages also display suppressive characteristics, which are addressed by the study of their hypostimulatory T lymphocyte capacity and polyclonal T lymphocyte activation suppression. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 9 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 2 | 22% |
Other | 1 | 11% |
Student > Doctoral Student | 1 | 11% |
Student > Master | 1 | 11% |
Researcher | 1 | 11% |
Other | 1 | 11% |
Unknown | 2 | 22% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 2 | 22% |
Neuroscience | 2 | 22% |
Immunology and Microbiology | 1 | 11% |
Biochemistry, Genetics and Molecular Biology | 1 | 11% |
Medicine and Dentistry | 1 | 11% |
Other | 1 | 11% |
Unknown | 1 | 11% |