| Chapter title |
Microglia Analysis in Retinal Degeneration Mouse Models
|
|---|---|
| Chapter number | 10 |
| Book title |
Mouse Retinal Phenotyping
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7720-8_10 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7719-2, 978-1-4939-7720-8
|
| Authors |
Katharina Dannhausen, Khalid Rashid, Thomas Langmann |
| Abstract |
Microgliosis is a hallmark of degenerative processes in the retina. Reactive microglia migrate to the photoreceptor layer and the subretinal space during outer retinal degeneration. This process creates a toxic milieu where reactive microglia and dying photoreceptors recruit additional reactive phagocytes. This results in the release of a multitude of proinflammatory factors which accelerate photoreceptor demise. In this chapter, we outline in detail how to monitor microgliosis in the Fam161a-deficient mouse model of Retinitis Pigmentosa by performing immunohistochemical stainings of retinal cryosections and flat mounts using the marker Iba1. This protocol will serve as a guideline in evaluating microglia reactivity and localization in various mouse models of retinal degeneration. |
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Demographic breakdown
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