Chapter title |
Morphological Identification of Melanopsin-Expressing Retinal Ganglion Cell Subtypes in Mice
|
---|---|
Chapter number | 19 |
Book title |
Mouse Retinal Phenotyping
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7720-8_19 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7719-2, 978-1-4939-7720-8
|
Authors |
Seul Ki Lee, Tiffany M. Schmidt, Lee, Seul Ki, Schmidt, Tiffany M. |
Abstract |
Melanopsin-expressing, intrinsically photosensitive retinal ganglion cells (ipRGCs) are a relatively recently discovered class of photoreceptor. ipRGCs can be subdivided into at least five subtypes (M1-M5), each of which has a distinct complement of morphological and physiological properties. ipRGC subtypes can be identified morphologically based on a combination of dendritic morphology and immunostaining for a cell-type specific marker. In this chapter, we describe methods for conclusively identifying each of the five ipRGC subtypes through a combination of patch clamp electrophysiology, Neurobiotin filling, visualization of ipRGC dendrites, and immunostaining for the marker SMI-32. |
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