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Mouse Retinal Phenotyping

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Cover of 'Mouse Retinal Phenotyping'

Table of Contents

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    Book Overview
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    Chapter 1 Morphological Survey from Neurons to Circuits of the Mouse Retina
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    Chapter 2 Measuring Retinal Function in the Mouse
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    Chapter 3 Modeling Retinal Diseases Using Genetic Approaches in Mice
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    Chapter 4 Cell Culture Analysis of the Phagocytosis of Photoreceptor Outer Segments by Primary Mouse RPE Cells
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    Chapter 5 Two-Photon Microscopy (TPM) and Fluorescence Lifetime Imaging Microscopy (FLIM) of Retinal Pigment Epithelium (RPE) of Mice In Vivo
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    Chapter 6 RPE Visual Cycle and Biochemical Phenotypes of Mutant Mouse Models
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    Chapter 7 Use of Direct Current Electroretinography for Analysis of Retinal Pigment Epithelium Function in Mouse Models
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    Chapter 8 Disruption of Rhodopsin Dimerization in Mouse Rod Photoreceptors by Synthetic Peptides Targeting Dimer Interface
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    Chapter 9 Experimental Approaches for Defining the Role of the Ca2+-Modulated ROS-GC System in Retinal Rods of Mouse
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    Chapter 10 Microglia Analysis in Retinal Degeneration Mouse Models
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    Chapter 11 Determination of Mitochondrial Oxygen Consumption in the Retina Ex Vivo: Applications for Retinal Disease
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    Chapter 12 Analysis of Feedback Signaling from Horizontal Cells to Photoreceptors in Mice
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    Chapter 13 Assessment of the Absolute Excitatory Level of the Retina by Flicker ERG
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    Chapter 14 Ex Vivo Functional Evaluation of Synaptic Transmission from Rods to Rod Bipolar Cells in Mice
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    Chapter 15 Functional and Morphological Analysis of OFF Bipolar Cells
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    Chapter 16 Immunohistochemical Phenotyping of Mouse Amacrine Cell Subtypes
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    Chapter 17 Phenotyping of Gap-Junctional Coupling in the Mouse Retina
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    Chapter 18 Ganglion Cell Assessment in Rodents with Retinal Degeneration
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    Chapter 19 Morphological Identification of Melanopsin-Expressing Retinal Ganglion Cell Subtypes in Mice
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    Chapter 20 Functional Assessment of Melanopsin-Driven Light Responses in the Mouse: Multielectrode Array Recordings
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    Chapter 21 In Vitro Assays for Mouse Müller Cell Phenotyping Through microRNA Profiling in the Damaged Retina
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    Chapter 22 Analysis of Retinal Vascular Plexuses and Interplexus Connections
Attention for Chapter 11: Determination of Mitochondrial Oxygen Consumption in the Retina Ex Vivo: Applications for Retinal Disease
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Chapter title
Determination of Mitochondrial Oxygen Consumption in the Retina Ex Vivo: Applications for Retinal Disease
Chapter number 11
Book title
Mouse Retinal Phenotyping
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7720-8_11
Pubmed ID
Book ISBNs
978-1-4939-7719-2, 978-1-4939-7720-8
Authors

Yogita K. Adlakha, Anand Swaroop

Abstract

Mitochondrial dysfunction, and consequently altered aerobic energy metabolism, is associated with numerous retinal diseases, including photoreceptor degeneration and diabetic retinopathy. Here, we describe a detailed protocol to directly measure oxygen consumption in the intact retina ex vivo using microplate-based fluorescence technology. We have used this method to assess preferred energy substrate for retinal tissue and suggested its application for investigating mechanisms of retinal disease.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 8 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 3 38%
Researcher 2 25%
Student > Bachelor 1 13%
Student > Doctoral Student 1 13%
Unknown 1 13%
Readers by discipline Count As %
Agricultural and Biological Sciences 2 25%
Neuroscience 2 25%
Nursing and Health Professions 1 13%
Medicine and Dentistry 1 13%
Biochemistry, Genetics and Molecular Biology 1 13%
Other 0 0%
Unknown 1 13%