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Mendeley readers
Chapter title |
Isolating Embryonic Cardiac Progenitors and Cardiac Myocytes by Fluorescence-Activated Cell Sorting
|
---|---|
Chapter number | 9 |
Book title |
Mouse Embryogenesis
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Published in |
Methods in molecular biology, January 2018
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DOI | 10.1007/978-1-4939-7714-7_9 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7713-0, 978-1-4939-7714-7
|
Authors |
Abdalla Ahmed, Paul Delgado-Olguin |
Abstract |
Isolation of highly purified populations of embryonic cardiomyocytes enables the study of congenital cardiac phenotypes at the cellular level. Fluorescent-activated cell sorting (FACS) is normally used to isolate fluorescently tagged cells. Here we describe the isolation of differentiating mouse embryonic cardiac progenitors and cardiomyocytes at embryonic day (E) 9.5 and E13.5, respectively by FACS. Over 50,000 differentiating cardiac progenitors and 200,000 cardiomyocytes can be obtained in a single prep using the methods described. |
Mendeley readers
The data shown below were compiled from readership statistics for 10 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 10 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Doctoral Student | 2 | 20% |
Student > Ph. D. Student | 2 | 20% |
Researcher | 2 | 20% |
Student > Master | 1 | 10% |
Student > Bachelor | 1 | 10% |
Other | 0 | 0% |
Unknown | 2 | 20% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 3 | 30% |
Agricultural and Biological Sciences | 2 | 20% |
Medicine and Dentistry | 1 | 10% |
Unknown | 4 | 40% |