| Chapter title |
Live Imaging of Fetal Intra-abdominal Organs Using Two-Photon Laser-Scanning Microscopy
|
|---|---|
| Chapter number | 6 |
| Book title |
Mouse Embryogenesis
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7714-7_6 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7713-0, 978-1-4939-7714-7
|
| Authors |
Yuhki Koike, Bo Li, Yong Chen, Hiromu Miyake, Carol Lee, Lijun Chi, Richard Wu, Mikihiro Inoue, Keiichi Uchida, Masato Kusunoki, Paul Delgado-Olguin, Agostino Pierro |
| Abstract |
The processes by which the intra-abdominal organ circulatory system develops in the embryo and during organogenesis are unclear. Previous studies have used fixed tissues to study the development of abdominal organ vasculature in the embryo; however, the intravital circulation of intra-abdominal organs in rodent fetal development has not been studied. This protocol describes a system that uses two-photon laser-scanning microscopy (TPLSM) for real-time observation and quantification of normal and pathologic live fetal intra-abdominal dynamics while the fetus is still connected to the mother via the umbilical cord. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 7 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 2 | 29% |
| Lecturer | 1 | 14% |
| Student > Postgraduate | 1 | 14% |
| Unknown | 3 | 43% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 1 | 14% |
| Immunology and Microbiology | 1 | 14% |
| Medicine and Dentistry | 1 | 14% |
| Neuroscience | 1 | 14% |
| Unknown | 3 | 43% |