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Mouse Embryogenesis

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Cover of 'Mouse Embryogenesis'

Table of Contents

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    Book Overview
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    Chapter 1 Mouse Genotyping
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    Chapter 2 Visualizing the Vascular Network in the Mouse Embryo and Yolk Sac
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    Chapter 3 In Vivo Evaluation of the Cardiovascular System of Mouse Embryo and Fetus Using High Frequency Ultrasound
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    Chapter 4 Dynamic Imaging of Mouse Embryos and Cardiodynamics in Static Culture
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    Chapter 5 In Vivo Imaging of the Mouse Reproductive Organs, Embryo Transfer, and Oviduct Cilia Dynamics Using Optical Coherence Tomography
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    Chapter 6 Live Imaging of Fetal Intra-abdominal Organs Using Two-Photon Laser-Scanning Microscopy
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    Chapter 7 Embryonary Mouse Cardiac Fibroblast Isolation
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    Chapter 8 Explant Culture for Studying Lung Development
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    Chapter 9 Isolating Embryonic Cardiac Progenitors and Cardiac Myocytes by Fluorescence-Activated Cell Sorting
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    Chapter 10 Isolation and Culture of Mouse Placental Endothelial Cells
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    Chapter 11 Flow Cytometry and Lineage Tracing Study for Identification of Adipocyte Precursor Cell (APC) Populations
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    Chapter 12 Whole-Mount and Section In Situ Hybridization in Mouse Embryos for Detecting mRNA Expression and Localization
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    Chapter 13 Chromosome Painting of Mouse Chromosomes
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    Chapter 14 Chromatin Immunoprecipitation in Early Mouse Embryos
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    Chapter 15 Shaping Up the Embryo: The Role of Genome 3D Organization
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    Chapter 16 Genome Editing During Development Using the CRISPR-Cas Technology
Attention for Chapter 2: Visualizing the Vascular Network in the Mouse Embryo and Yolk Sac
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Chapter title
Visualizing the Vascular Network in the Mouse Embryo and Yolk Sac
Chapter number 2
Book title
Mouse Embryogenesis
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7714-7_2
Pubmed ID
Book ISBNs
978-1-4939-7713-0, 978-1-4939-7714-7
Authors

Anna R. Roy, Paul Delgado-Olguin

Abstract

Whole mount immunofluorescence is a valuable technique that can be used to visualize vascular networks in early developing embryonic tissues. This technique involves the permeabilization of fixed mouse embryos and yolk sacs, and primary antibody tagging of the endothelial cell marker platelet endothelial cell adhesion molecule 1 (Pecam-1). A secondary antibody tagged with a fluorophore targets the primary antibody, fluorescently labeling endothelial cells and revealing vascular networks.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 9 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 9 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 2 22%
Student > Bachelor 2 22%
Student > Doctoral Student 1 11%
Student > Master 1 11%
Unknown 3 33%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 4 44%
Agricultural and Biological Sciences 2 22%
Unknown 3 33%