| Chapter title |
Flow Cytometry and Lineage Tracing Study for Identification of Adipocyte Precursor Cell (APC) Populations
|
|---|---|
| Chapter number | 11 |
| Book title |
Mouse Embryogenesis
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7714-7_11 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7713-0, 978-1-4939-7714-7
|
| Authors |
Ju Hee Lee, Azadeh Yeganeh, Hisato Konoeda, Joon Ho Moon, Hoon-Ki Sung |
| Abstract |
Flow cytometry and fluorescence-activated cell sorting (FACS) techniques have significantly advanced the characterization of adipocyte precursor cell (APC) populations. They allow immunophenotyping, quantification, and isolation of distinct populations, which is critical for understanding adipose tissue development and homeostasis. Here, we describe the identification and purification of adipocyte precursor cells using flow cytometry and FACS, defined by previously established surface marker profiles. In addition, we describe the mouse models and whole adipose tissue visualization techniques that will enable us to characterize the plasticity and the cellular origin of APCs. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 14 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 2 | 14% |
| Student > Ph. D. Student | 2 | 14% |
| Unspecified | 1 | 7% |
| Unknown | 9 | 64% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemical Engineering | 1 | 7% |
| Unspecified | 1 | 7% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 7% |
| Biochemistry, Genetics and Molecular Biology | 1 | 7% |
| Unknown | 10 | 71% |