Chapter title |
Microfluidic Devices for Examining the Physical Limits of Migration in Confined Environments
|
---|---|
Chapter number | 27 |
Book title |
Cell Migration
|
Published in |
Methods in molecular biology, March 2018
|
DOI | 10.1007/978-1-4939-7701-7_27 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7700-0, 978-1-4939-7701-7
|
Authors |
Majid Malboubi, Asier Jayo, Maddy Parsons, Guillaume Charras, Malboubi, M, Jayo, A, Parsons, M, Charras, G |
Abstract |
Cell migration plays a key role in many physiological and pathological conditions during which cells migrate primarily in the 3D environments formed by tissues. Microfluidics enables the design of simple devices that can mimic in a highly controlled manner the geometry and dimensions of the interstices encountered by cells in the body. Here we describe the design, fabrication, and implementation of an array of channels with a range of cross sections to investigate migration of cells and cell clusters through confined spaces. By combining this assay with a motorized microscope stage, image data can be acquired with high throughput to determine the physical limits of migration in confined environments and their biological origin. |
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