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Cell Migration

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Cover of 'Cell Migration'

Table of Contents

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    Book Overview
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    Chapter 1 Random Migration Assays of Mammalian Cells and Quantitative Analyses of Single Cell Trajectories
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    Chapter 2 Directional Collective Migration in Wound Healing Assays
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    Chapter 3 An In Vitro System to Study the Mesenchymal-to-Amoeboid Transition
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    Chapter 4 An In Vitro System to Study the Epithelial–Mesenchymal Transition In Vitro
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    Chapter 5 Detection of Migrasomes
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    Chapter 6 3D Endothelial Cell Migration
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    Chapter 7 Transmigration of Leukocytes Across Epithelial Monolayers
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    Chapter 8 Evaluation of Tumor Cell Invasiveness In Vivo: The Chick Chorioallantoic Membrane Assay
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    Chapter 9 Analysis of Invasion Dynamics of Matrix-Embedded Cells in a Multisample Format
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    Chapter 10 Using Systems Microscopy to Understand the Emergence of Cell Migration from Cell Organization
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    Chapter 11 Neuronal Precursor Migration in Ex Vivo Brain Slice Culture
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    Chapter 12 In Vitro Models to Analyze the Migration of MGE-Derived Interneurons
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    Chapter 13 Cell Migration in Tissues: Explant Culture and Live Imaging
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    Chapter 14 Intravital Imaging of Tumor Cell Motility in the Tumor Microenvironment Context
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    Chapter 15 Using the Zebrafish Embryo to Dissect the Early Steps of the Metastasis Cascade
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    Chapter 16 Analysis of In Vivo Cell Migration in Mosaic Zebrafish Embryos
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    Chapter 17 Analysis of Cell Shape and Cell Migration of Drosophila Macrophages In Vivo
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    Chapter 18 Migration of Q Cells in Caenorhabditis elegans
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    Chapter 19 Imaging the Molecular Machines That Power Cell Migration
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    Chapter 20 A Biologist-Friendly Method to Analyze Cross-Correlation Between Protrusion Dynamics and Membrane Recruitment of Actin Regulators
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    Chapter 21 Using Single-Protein Tracking to Study Cell Migration
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    Chapter 22 Optogenetic Control of Cell Migration
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    Chapter 23 Electrotaxis: Cell Directional Movement in Electric Fields
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    Chapter 24 Analysis of Random Migration of Dictyostelium Amoeba in Confined and Unconfined Environments
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    Chapter 25 Neutrophil Chemotaxis in One Droplet of Blood Using Microfluidic Assays
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    Chapter 26 Leukocyte Migration and Deformation in Collagen Gels and Microfabricated Constrictions
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    Chapter 27 Microfluidic Devices for Examining the Physical Limits of Migration in Confined Environments
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    Chapter 28 Controlling Confinement and Topology to Study Collective Cell Behaviors
Attention for Chapter 20: A Biologist-Friendly Method to Analyze Cross-Correlation Between Protrusion Dynamics and Membrane Recruitment of Actin Regulators
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Chapter title
A Biologist-Friendly Method to Analyze Cross-Correlation Between Protrusion Dynamics and Membrane Recruitment of Actin Regulators
Chapter number 20
Book title
Cell Migration
Published in
Methods in molecular biology, March 2018
DOI 10.1007/978-1-4939-7701-7_20
Pubmed ID
Book ISBNs
978-1-4939-7700-0, 978-1-4939-7701-7
Authors

Perrine Paul-Gilloteaux, François Waharte, Manish Kumar Singh, Maria Carla Parrini

Abstract

During mesenchymal cell motility, various actin regulators are recruited to the leading edge with exquisite precision in time and space to generate protrusion and retraction cycles. We present here an automated method, named CorRecD (from Correlation Recruitment Dynamics), which quantifies cell edge dynamics, protein recruitment and analyze their cross-correlation. The Wave Regulatory Complex (WRC), a master driver of protrusions, is used as a case-of-study. This biologist-friendly method relies on free software tools and can be applied to any fluorescently tagged protein of interest.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 6 100%

Demographic breakdown

Readers by professional status Count As %
Professor > Associate Professor 1 17%
Researcher 1 17%
Lecturer > Senior Lecturer 1 17%
Unknown 3 50%
Readers by discipline Count As %
Mathematics 1 17%
Biochemistry, Genetics and Molecular Biology 1 17%
Unknown 4 67%