Chapter title |
Controlling Confinement and Topology to Study Collective Cell Behaviors
|
---|---|
Chapter number | 28 |
Book title |
Cell Migration
|
Published in |
Methods in molecular biology, March 2018
|
DOI | 10.1007/978-1-4939-7701-7_28 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7700-0, 978-1-4939-7701-7
|
Authors |
Duclos, Guillaume, Deforet, Maxime, Yevick, Hannah G., Cochet-Escartin, Olivier, Ascione, Flora, Moitrier, Sarah, Sarkar, Trinish, Yashunsky, Victor, Bonnet, Isabelle, Buguin, Axel, Silberzan, Pascal, Guillaume Duclos, Maxime Deforet, Hannah G. Yevick, Olivier Cochet-Escartin, Flora Ascione, Sarah Moitrier, Trinish Sarkar, Victor Yashunsky, Isabelle Bonnet, Axel Buguin, Pascal Silberzan |
Abstract |
Confinement and substrate topology strongly affect the behavior of cell populations and, in particular, their collective migration. In vitro experiments dealing with these aspects require strategies of surface patterning that remain effective over long times (typically several days) and ways to control the surface topology in three dimensions. Here, we describe protocols addressing these two aspects. High-resolution patterning of a robust cell-repellent coating is achieved by etching the coating through a photoresist mask patterned directly on the coated surface. Out-of-plane curvature can be controlled using glass wires or corrugated "wavy" surfaces. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 19 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 5 | 26% |
Student > Postgraduate | 4 | 21% |
Student > Bachelor | 2 | 11% |
Student > Master | 2 | 11% |
Researcher | 2 | 11% |
Other | 2 | 11% |
Unknown | 2 | 11% |
Readers by discipline | Count | As % |
---|---|---|
Physics and Astronomy | 7 | 37% |
Engineering | 3 | 16% |
Biochemistry, Genetics and Molecular Biology | 2 | 11% |
Nursing and Health Professions | 1 | 5% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 5% |
Other | 2 | 11% |
Unknown | 3 | 16% |