| Chapter title |
Leukocyte Migration and Deformation in Collagen Gels and Microfabricated Constrictions
|
|---|---|
| Chapter number | 26 |
| Book title |
Cell Migration
|
| Published in |
Methods in molecular biology, March 2018
|
| DOI | 10.1007/978-1-4939-7701-7_26 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7700-0, 978-1-4939-7701-7
|
| Authors |
Pablo J. Sáez, Lucie Barbier, Rafaele Attia, Hawa-Racine Thiam, Matthieu Piel, Pablo Vargas, Sáez, Pablo J., Barbier, Lucie, Attia, Rafaele, Thiam, Hawa-Racine, Piel, Matthieu, Vargas, Pablo |
| Abstract |
In multicellular organisms, cell migration is a complex process. Examples of this are observed during cell motility in the interstitial space, full of extracellular matrix fibers, or when cells pass through endothelial layers to colonize or exit specific tissues. A common parameter for both situations is the fast adaptation of the cellular shape to their irregular landscape. In this chapter, we describe two methods to study cell migration in complex environments. The first one consists in a multichamber device for the visualization of cell haptotaxis toward the collagen-binding chemokine CCL21. This method is used to study cell migration as well as deformations during directed motility, as in the interstitial space. The second one consists in microfabricated channels connected to small constrictions. This procedure allows the study of cell deformations when single cells migrate through small holes and it is analogous to passage of cells through endothelial layers, resulting in a simplified system to study the mechanisms operating during transvasation. Both methods combined provide a powerful hub for the study of cell plasticity during migration in complex environments. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 1 | 50% |
| United Kingdom | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 50% |
| Science communicators (journalists, bloggers, editors) | 1 | 50% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 26 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 6 | 23% |
| Student > Ph. D. Student | 6 | 23% |
| Student > Bachelor | 4 | 15% |
| Student > Master | 2 | 8% |
| Other | 1 | 4% |
| Other | 2 | 8% |
| Unknown | 5 | 19% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 11 | 42% |
| Agricultural and Biological Sciences | 3 | 12% |
| Immunology and Microbiology | 2 | 8% |
| Physics and Astronomy | 1 | 4% |
| Neuroscience | 1 | 4% |
| Other | 1 | 4% |
| Unknown | 7 | 27% |