Chapter title |
Transmigration of Leukocytes Across Epithelial Monolayers
|
---|---|
Chapter number | 7 |
Book title |
Cell Migration
|
Published in |
Methods in molecular biology, March 2018
|
DOI | 10.1007/978-1-4939-7701-7_7 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7700-0, 978-1-4939-7701-7
|
Authors |
Penny E. Morton, Maddy Parsons |
Abstract |
Migration of leukocytes through epithelial monolayers represents an essential step in the generation of an inflammatory response and is often seen in inflammatory conditions such as Crohn's disease (Matthews et al., Toxicol Pathol 42:91-98, 2014) and asthma (Lambrecht and Hammad, Nat Med 18:684-692, 2012). Transepithelial migration involves adhesion to the basal surface of the epithelium before migration through the epithelial cell layer to the apical surface. Analyzing this process can present a technical challenge due to complications of using a coculture model and trying to recapitulate an intact monolayer. Here we describe two methods of assessing transepithelial migration based on a Transwell assay, the first of which measures the apical-basal migration of epithelial cells and the second "Inverted" transwell assay that measures basal-apical transmigration of leukocytes and therefore more closely mimics the in vivo process. |
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Geographical breakdown
Country | Count | As % |
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Unknown | 5 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 1 | 20% |
Professor | 1 | 20% |
Librarian | 1 | 20% |
Student > Doctoral Student | 1 | 20% |
Unknown | 1 | 20% |
Readers by discipline | Count | As % |
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Engineering | 2 | 40% |
Unknown | 1 | 20% |