| Chapter title |
Techniques to Analyze sRNA Protein Cofactor Self-Assembly In Vitro
|
|---|---|
| Chapter number | 18 |
| Book title |
Bacterial Regulatory RNA
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7634-8_18 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7633-1, 978-1-4939-7634-8
|
| Authors |
David Partouche, Antoine Malabirade, Thomas Bizien, Marisela Velez, Sylvain Trépout, Sergio Marco, Valeria Militello, Christophe Sandt, Frank Wien, Véronique Arluison |
| Abstract |
Post-transcriptional control of gene expression by small regulatory noncoding RNA (sRNA) needs protein accomplices to occur. Past research mainly focused on the RNA chaperone Hfq as cofactor. Nevertheless, recent studies indicated that other proteins might be involved in sRNA-based regulations. As some of these proteins have been shown to self-assemble, we describe in this chapter protocols to analyze the nano-assemblies formed. Precisely, we focus our analysis on Escherichia coli Hfq as a model, but the protocols presented here can be applied to analyze any polymer of proteins. This chapter thus provides a guideline to develop commonly used approaches to detect prokaryotic protein self-assembly, with a special focus on the detection of amyloidogenic polymers. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 10 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 4 | 40% |
| Student > Ph. D. Student | 2 | 20% |
| Professor > Associate Professor | 1 | 10% |
| Unknown | 3 | 30% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 2 | 20% |
| Chemistry | 2 | 20% |
| Physics and Astronomy | 1 | 10% |
| Agricultural and Biological Sciences | 1 | 10% |
| Unknown | 4 | 40% |