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Mendeley readers
Chapter title |
Evaluating the Effect of Small RNAs and Associated Chaperones on Rho-Dependent Termination of Transcription In Vitro
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Chapter number | 7 |
Book title |
Bacterial Regulatory RNA
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Published in |
Methods in molecular biology, January 2018
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DOI | 10.1007/978-1-4939-7634-8_7 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7633-1, 978-1-4939-7634-8
|
Authors |
Cédric Nadiras, Annie Schwartz, Mildred Delaleau, Marc Boudvillain |
Abstract |
Besides their well-known posttranscriptional effects on mRNA translation and decay, sRNAs and associated RNA chaperones (e.g., Hfq, CsrA) sometimes regulate gene expression at the transcriptional level. In this case, the sRNA-dependent machinery modulates the activity of the transcription termination factor Rho, a ring-shaped RNA translocase/helicase that dissociates transcription elongation complexes at specific loci of the bacterial genome. Here, we describe biochemical assays to detect Rho-dependent termination signals in genomic regions of interest and to assess the effects of sRNAs and/or associated RNA chaperones on such signals. |
Mendeley readers
The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 7 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 2 | 29% |
Student > Bachelor | 2 | 29% |
Researcher | 2 | 29% |
Student > Master | 1 | 14% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 4 | 57% |
Agricultural and Biological Sciences | 1 | 14% |
Immunology and Microbiology | 1 | 14% |
Unknown | 1 | 14% |