Chapter title |
Single-Molecule FRET Assay to Observe the Activity of Proteins Involved in RNA/RNA Annealing
|
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Chapter number | 17 |
Book title |
Bacterial Regulatory RNA
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7634-8_17 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7633-1, 978-1-4939-7634-8
|
Authors |
Thierry Bizebard, Véronique Arluison, Ulrich Bockelmann |
Abstract |
In recent years, single-molecule fluorescence resonance energy transfer (smFRET) has emerged as a powerful technique to study macromolecular interactions. The chief advantages of smFRET analysis compared to bulk measurements include the possibility to detect sample heterogeneities within a large population of molecules and the facility to measure kinetics without needing the synchronization of intermediate states. As such, the methodology is particularly well adapted to observe and analyze RNA/RNA and RNA/protein interactions involved in small noncoding RNA-mediated gene regulation networks. In this chapter, we describe and discuss protocols that can be used to measure the dynamics of these interactions, with a particular emphasis on the advantages-and experimental pitfalls-of using the smFRET methodology to study sRNA-based biological systems. |
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Unknown | 5 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 3 | 60% |
Student > Doctoral Student | 1 | 20% |
Unknown | 1 | 20% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 3 | 60% |
Physics and Astronomy | 1 | 20% |
Unknown | 1 | 20% |