Chapter title |
Sequence-Specific Affinity Chromatography of Bacterial Small Regulatory RNA-Binding Proteins from Bacterial Cells
|
---|---|
Chapter number | 19 |
Book title |
Bacterial Regulatory RNA
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7634-8_19 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7633-1, 978-1-4939-7634-8
|
Authors |
Jonathan Gans, Jonathan Osborne, Juliet Cheng, Louise Djapgne, Amanda G. Oglesby-Sherrouse, Gans, Jonathan, Osborne, Jonathan, Cheng, Juliet, Djapgne, Louise, Oglesby-Sherrouse, Amanda G. |
Abstract |
Bacterial small RNA molecules (sRNAs) are increasingly recognized as central regulators of bacterial stress responses and pathogenesis. In many cases, RNA-binding proteins are critical for the stability and function of sRNAs. Previous studies have adopted strategies to genetically tag an sRNA of interest, allowing isolation of RNA-protein complexes from cells. Here we present a sequence-specific affinity purification protocol that requires no prior genetic manipulation of bacterial cells, allowing isolation of RNA-binding proteins bound to native RNA molecules. |
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