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Chapter title |
RNA Tagging: Preparation of High-Throughput Sequencing Libraries
|
---|---|
Chapter number | 30 |
Book title |
RNA Detection
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7213-5_30 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7212-8, 978-1-4939-7213-5
|
Authors |
Christopher P. Lapointe, Marvin Wickens |
Abstract |
Protein-RNA networks, in which a single protein binds and controls multiple mRNAs, are central in biological control. As a result, methods to identify protein-RNA interactions that occur in vivo are valuable. The "RNA Tagging" approach enables the investigator to unambiguously identify global protein-RNA interactions in vivo and is independent of protein purification, cross-linking, and radioactive labeling steps. Here, we provide a protocol to prepare high-throughput sequencing libraries for RNA Tagging experiments. |
Mendeley readers
The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 7 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 4 | 57% |
Student > Ph. D. Student | 2 | 29% |
Unknown | 1 | 14% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 2 | 29% |
Agricultural and Biological Sciences | 2 | 29% |
Immunology and Microbiology | 1 | 14% |
Unknown | 2 | 29% |