Chapter title |
Real-Time Fluorescence Imaging of Single-Molecule Endogenous Noncoding RNA in Living Cells
|
---|---|
Chapter number | 22 |
Book title |
RNA Detection
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7213-5_22 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7212-8, 978-1-4939-7213-5
|
Authors |
Hideaki Yoshimura, Takeaki Ozawa |
Abstract |
Visualizing RNA in living cells is increasingly important to facilitate accumulation of knowledge about the relation between specific RNA dynamics and physiological events. Single-molecule fluorescence imaging of target RNAs is an excellent approach to analyzing intracellular RNA motion, but it requires special techniques for probe design and microscope setup. Herein, we present a principle and protocol of an RNA visualization probe based on an RNA binding protein of the Pumilio homology domain (PUM-HD). We also describe the setup and operation of a microscope, and introduce an application to visualize telomeric repeats-containing RNA with telomeres and a telomere-related protein: hnRNPA1. This imaging technique is applicable to visualization of different RNAs, especially including repetitive sequences, in living cells. |
Mendeley readers
Geographical breakdown
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Unknown | 9 | 100% |
Demographic breakdown
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Student > Ph. D. Student | 3 | 33% |
Student > Master | 2 | 22% |
Professor | 1 | 11% |
Other | 1 | 11% |
Researcher | 1 | 11% |
Other | 0 | 0% |
Unknown | 1 | 11% |
Readers by discipline | Count | As % |
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Chemistry | 2 | 22% |
Neuroscience | 1 | 11% |
Medicine and Dentistry | 1 | 11% |
Unknown | 2 | 22% |