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RNA Detection

Overview of attention for book
Cover of 'RNA Detection'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 The Secret Life of RNA: Lessons from Emerging Methodologies
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    Chapter 2 Quantification of 2′-O-Me Residues in RNA Using Next-Generation Sequencing (Illumina RiboMethSeq Protocol)
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    Chapter 3 Identifying the m6A Methylome by Affinity Purification and Sequencing
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    Chapter 4 PARIS: Psoralen Analysis of RNA Interactions and Structures with High Throughput and Resolution
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    Chapter 5 Axon-TRAP-RiboTag: Affinity Purification of Translated mRNAs from Neuronal Axons in Mouse In Vivo
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    Chapter 6 LCM-Seq: A Method for Spatial Transcriptomic Profiling Using Laser Capture Microdissection Coupled with PolyA-Based RNA Sequencing
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    Chapter 7 Spatial Transcriptomics: Constructing a Single-Cell Resolution Transcriptome-Wide Expression Atlas
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    Chapter 8 Single mRNA Molecule Detection in Drosophila
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    Chapter 9 Detection and Automated Analysis of Single Transcripts at Subcellular Resolution in Zebrafish Embryos
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    Chapter 10 Super-Resolution Single Molecule FISH at the Drosophila Neuromuscular Junction
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    Chapter 11 Detection of mRNA and Associated Molecules by ISH-IEM on Frozen Sections
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    Chapter 12 Hybridization Chain Reaction for Direct mRNA Detection Without Nucleic Acid Purification
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    Chapter 13 In Situ Detection of MicroRNA Expression with RNAscope Probes
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    Chapter 14 Padlock Probes to Detect Single Nucleotide Polymorphisms
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    Chapter 15 Quantifying Gene Expression in Living Cells with Ratiometric Bimolecular Beacons
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    Chapter 16 Optimizing Molecular Beacons for Intracellular Analysis of RNA
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    Chapter 17 Live Imaging of Nuclear RNPs in Mammalian Complex Tissue with ECHO-liveFISH
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    Chapter 18 In Vivo Visualization and Function Probing of Transport mRNPs Using Injected FIT Probes
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    Chapter 19 Visualizing RNA in Live Bacterial Cells Using Fluorophore- and Quencher-Binding Aptamers
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    Chapter 20 Method for Imaging Live-Cell RNA Using an RNA Aptamer and a Fluorescent Probe
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    Chapter 21 RNA Live Imaging in the Model Microorganism Ustilago maydis
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    Chapter 22 Real-Time Fluorescence Imaging of Single-Molecule Endogenous Noncoding RNA in Living Cells
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    Chapter 23 Live Imaging of mRNA Synthesis in Drosophila
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    Chapter 24 Imaging Newly Transcribed RNA in Cells by Using a Clickable Azide-Modified UTP Analog
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    Chapter 25 Detection of the First Round of Translation: The TRICK Assay
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    Chapter 26 Imaging Translation Dynamics of Single mRNA Molecules in Live Cells
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    Chapter 27 Systematic Detection of Poly(A)+ RNA-Interacting Proteins and Their Differential Binding
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    Chapter 28 Isolation and Characterization of Endogenous RNPs from Brain Tissues
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    Chapter 29 Individual Nucleotide Resolution UV Cross-Linking and Immunoprecipitation (iCLIP) to Determine Protein–RNA Interactions
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    Chapter 30 RNA Tagging: Preparation of High-Throughput Sequencing Libraries
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    Chapter 31 RAP-MS: A Method to Identify Proteins that Interact Directly with a Specific RNA Molecule in Cells
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    Chapter 32 Erratum to: Super-Resolution Single Molecule FISH at the Drosophila Neuromuscular Junction
Attention for Chapter 23: Live Imaging of mRNA Synthesis in Drosophila
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Chapter title
Live Imaging of mRNA Synthesis in Drosophila
Chapter number 23
Book title
RNA Detection
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7213-5_23
Pubmed ID
Book ISBNs
978-1-4939-7212-8, 978-1-4939-7213-5
Authors

Hernan G. Garcia, Thomas Gregor

Abstract

mRNA synthesis is one of the earliest readouts of the activity of a transcribed gene, which is of particular interest in the context of metazoan cell fate specification. These processes are intrinsically dynamic and stochastic, which makes in vivo single-cell measurements inevitable. Here, we present the application of a technology that has been widely used in single celled organisms to measure transcriptional activity in developing embryos of the fruit fly Drosophila melanogaster. The method allows for quantification of instantaneous polymerase occupancy of active gene loci and thereby enables the development and testing of models of gene regulation in development.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 35 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 35 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 9 26%
Student > Ph. D. Student 9 26%
Student > Bachelor 7 20%
Student > Doctoral Student 3 9%
Professor 3 9%
Other 3 9%
Unknown 1 3%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 21 60%
Agricultural and Biological Sciences 4 11%
Physics and Astronomy 4 11%
Business, Management and Accounting 2 6%
Psychology 1 3%
Other 0 0%
Unknown 3 9%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 November 2017.
All research outputs
#18,345,259
of 23,577,654 outputs
Outputs from Methods in molecular biology
#7,519
of 13,410 outputs
Outputs of similar age
#313,736
of 444,928 outputs
Outputs of similar age from Methods in molecular biology
#864
of 1,483 outputs
Altmetric has tracked 23,577,654 research outputs across all sources so far. This one is in the 19th percentile – i.e., 19% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,410 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 38th percentile – i.e., 38% of its peers scored the same or lower than it.
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We're also able to compare this research output to 1,483 others from the same source and published within six weeks on either side of this one. This one is in the 36th percentile – i.e., 36% of its contemporaries scored the same or lower than it.