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RNA Detection

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Cover of 'RNA Detection'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 The Secret Life of RNA: Lessons from Emerging Methodologies
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    Chapter 2 Quantification of 2′-O-Me Residues in RNA Using Next-Generation Sequencing (Illumina RiboMethSeq Protocol)
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    Chapter 3 Identifying the m6A Methylome by Affinity Purification and Sequencing
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    Chapter 4 PARIS: Psoralen Analysis of RNA Interactions and Structures with High Throughput and Resolution
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    Chapter 5 Axon-TRAP-RiboTag: Affinity Purification of Translated mRNAs from Neuronal Axons in Mouse In Vivo
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    Chapter 6 LCM-Seq: A Method for Spatial Transcriptomic Profiling Using Laser Capture Microdissection Coupled with PolyA-Based RNA Sequencing
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    Chapter 7 Spatial Transcriptomics: Constructing a Single-Cell Resolution Transcriptome-Wide Expression Atlas
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    Chapter 8 Single mRNA Molecule Detection in Drosophila
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    Chapter 9 Detection and Automated Analysis of Single Transcripts at Subcellular Resolution in Zebrafish Embryos
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    Chapter 10 Super-Resolution Single Molecule FISH at the Drosophila Neuromuscular Junction
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    Chapter 11 Detection of mRNA and Associated Molecules by ISH-IEM on Frozen Sections
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    Chapter 12 Hybridization Chain Reaction for Direct mRNA Detection Without Nucleic Acid Purification
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    Chapter 13 In Situ Detection of MicroRNA Expression with RNAscope Probes
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    Chapter 14 Padlock Probes to Detect Single Nucleotide Polymorphisms
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    Chapter 15 Quantifying Gene Expression in Living Cells with Ratiometric Bimolecular Beacons
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    Chapter 16 Optimizing Molecular Beacons for Intracellular Analysis of RNA
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    Chapter 17 Live Imaging of Nuclear RNPs in Mammalian Complex Tissue with ECHO-liveFISH
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    Chapter 18 In Vivo Visualization and Function Probing of Transport mRNPs Using Injected FIT Probes
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    Chapter 19 Visualizing RNA in Live Bacterial Cells Using Fluorophore- and Quencher-Binding Aptamers
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    Chapter 20 Method for Imaging Live-Cell RNA Using an RNA Aptamer and a Fluorescent Probe
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    Chapter 21 RNA Live Imaging in the Model Microorganism Ustilago maydis
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    Chapter 22 Real-Time Fluorescence Imaging of Single-Molecule Endogenous Noncoding RNA in Living Cells
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    Chapter 23 Live Imaging of mRNA Synthesis in Drosophila
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    Chapter 24 Imaging Newly Transcribed RNA in Cells by Using a Clickable Azide-Modified UTP Analog
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    Chapter 25 Detection of the First Round of Translation: The TRICK Assay
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    Chapter 26 Imaging Translation Dynamics of Single mRNA Molecules in Live Cells
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    Chapter 27 Systematic Detection of Poly(A)+ RNA-Interacting Proteins and Their Differential Binding
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    Chapter 28 Isolation and Characterization of Endogenous RNPs from Brain Tissues
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    Chapter 29 Individual Nucleotide Resolution UV Cross-Linking and Immunoprecipitation (iCLIP) to Determine Protein–RNA Interactions
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    Chapter 30 RNA Tagging: Preparation of High-Throughput Sequencing Libraries
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    Chapter 31 RAP-MS: A Method to Identify Proteins that Interact Directly with a Specific RNA Molecule in Cells
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    Chapter 32 Erratum to: Super-Resolution Single Molecule FISH at the Drosophila Neuromuscular Junction
Attention for Chapter 21: RNA Live Imaging in the Model Microorganism Ustilago maydis
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Chapter title
RNA Live Imaging in the Model Microorganism Ustilago maydis
Chapter number 21
Book title
RNA Detection
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7213-5_21
Pubmed ID
Book ISBNs
978-1-4939-7212-8, 978-1-4939-7213-5
Authors

Sabrina Zander, Kira Müntjes, Michael Feldbrügge

Abstract

An essential feature of protein expression is the tight regulation of when and where a protein is translated from its cognate mRNA. This spatiotemporal expression is particularly important in guaranteeing the correct and efficient targeting of proteins to defined subcellular sites. In order to achieve local translation, mRNAs must be deposited at specific locations. A common mechanism is the active transport of mRNAs along the actin or microtubule cytoskeleton. To study such dynamic transport processes in vivo RNA live imaging is the method of choice. This method is based on the principle that defined binding sites for a heterologous RNA-binding protein (RBP) are inserted in the 3' UTR of target mRNAs. Coexpression of the RBP fused to a fluorescent protein enables mRNA detection in vivo using fluorescence microscopy techniques. In this chapter we describe the well-established method of studying microtubule-dependent mRNA transport in the eukaryotic model microorganism Ustilago maydis. The presented experimental design and the microscopic techniques are applicable to a broad range of other organisms.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 3 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 3 100%

Demographic breakdown

Readers by professional status Count As %
Student > Postgraduate 1 33%
Other 1 33%
Unknown 1 33%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 1 33%
Agricultural and Biological Sciences 1 33%
Unknown 1 33%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 November 2017.
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#18,576,855
of 23,008,860 outputs
Outputs from Methods in molecular biology
#7,962
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#330,487
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Outputs of similar age from Methods in molecular biology
#950
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