Chapter title |
Nano/Micro Formulations for Bacteriophage Delivery
|
---|---|
Chapter number | 20 |
Book title |
Bacteriophage Therapy
|
Published in |
Methods in molecular biology, November 2017
|
DOI | 10.1007/978-1-4939-7395-8_20 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7394-1, 978-1-4939-7395-8
|
Authors |
Pilar Cortés, Mary Cano-Sarabia, Joan Colom, Jennifer Otero, Daniel Maspoch, Montserrat Llagostera, Cortés, Pilar, Cano-Sarabia, Mary, Colom, Joan, Otero, Jennifer, Maspoch, Daniel, Llagostera, Montserrat |
Abstract |
Encapsulation methodologies allow the protection of bacteriophages for overcoming critical environmental conditions. Moreover, they improve the stability and the controlled delivery of bacteriophages which is of great innovative value in bacteriophage therapy. Here, two different encapsulation methodologies of bacteriophages are described using two biocompatible materials: a lipid cationic mixture and a combination of alginate with the antacid CaCO3. To perform bacteriophage encapsulation, a purified lysate highly concentrated (around 10(10)-10(11) pfu/mL) is necessary, and to dispose of a specific equipment. Both methodologies have been successfully applied for encapsulating Salmonella bacteriophages with different morphologies. Also, the material employed does not modify the antibacterial action of bacteriophages. Moreover, both technologies can also be adapted to any bacteriophage and possibly to any delivery route for bacteriophage therapy. |
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