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Bacteriophage Therapy

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Cover of 'Bacteriophage Therapy'

Table of Contents

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    Book Overview
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    Chapter 1 Isolation of Bacteriophages for Fastidious Bacteria
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    Chapter 2 Isolation of Bacteriophages of the Anaerobic Bacteria Bacteroides
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    Chapter 3 Isolation of Bacteriophages for Clinically Relevant Bacteria
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    Chapter 4 In Vitro Activity of Bacteriophages Against Planktonic and Biofilm Populations Assessed by Flow Cytometry
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    Chapter 5 Observation of Bacteriophage Ultrastructure by Cryo-electron Microscopy
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    Chapter 6 Bacteriophage Taxonomy: An Evolving Discipline
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    Chapter 7 Determination of the Bacteriophage Host Range: Culture-Based Approach
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    Chapter 8 Recovery and Characterization of Bacteria Resisting Infection by Lytic Bacteriophage
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    Chapter 9 Guidelines to Compose an Ideal Bacteriophage Cocktail
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    Chapter 10 Interaction of Bacteriophages with Mammalian Cells
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    Chapter 11 In Vivo Bacteriophage Biodistribution
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    Chapter 12 Interaction of Bacteriophages with the Immune System: Induction of Bacteriophage-Specific Antibodies
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    Chapter 13 Bacteriophage Treatment of Infected Diabetic Foot Ulcers
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    Chapter 14 Compassionate Use of Bacteriophage Therapy for Foot Ulcer Treatment as an Effective Step for Moving Toward Clinical Trials
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    Chapter 15 Bacteriophage Production in Bioreactors
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    Chapter 16 Computational Modeling of Bacteriophage Production for Process Optimization
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    Chapter 17 Methods for Bacteriophage Preservation
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    Chapter 18 Bacteriophage Production in Compliance with Regulatory Requirements
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    Chapter 19 Guidelines for Bacteriophage Product Certification
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    Chapter 20 Nano/Micro Formulations for Bacteriophage Delivery
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    Chapter 21 Synthetic Biology to Engineer Bacteriophage Genomes
Attention for Chapter 2: Isolation of Bacteriophages of the Anaerobic Bacteria Bacteroides
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Chapter title
Isolation of Bacteriophages of the Anaerobic Bacteria Bacteroides
Chapter number 2
Book title
Bacteriophage Therapy
Published in
Methods in molecular biology, November 2017
DOI 10.1007/978-1-4939-7395-8_2
Pubmed ID
Book ISBNs
978-1-4939-7394-1, 978-1-4939-7395-8
Authors

Cristina García-Aljaro, Maite Muniesa, Juan Jofre

Abstract

Here we describe the detection, enumeration, and isolation of bacteriophages infecting Bacteroides. The method is based on the infection of Bacteroides host strains and the production of visible plaques in a confluent lawn of the host strain using the double-layer agar method. This is a straightforward methodology that can be applied for the detection, enumeration and isolation of bacteriophages for other anaerobic bacteria, using an appropriate host strain and culture conditions. In the case of bacteriophages of Bacteroides the results can be obtained in less than 24 h, although the time could vary depending on the growth rate of the host strain.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 19 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 19 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 4 21%
Researcher 4 21%
Student > Ph. D. Student 2 11%
Professor > Associate Professor 2 11%
Student > Bachelor 2 11%
Other 1 5%
Unknown 4 21%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 8 42%
Immunology and Microbiology 6 32%
Agricultural and Biological Sciences 1 5%
Unknown 4 21%