| Chapter title |
Bacteriophage Production in Bioreactors
|
|---|---|
| Chapter number | 15 |
| Book title |
Bacteriophage Therapy
|
| Published in |
Methods in molecular biology, November 2017
|
| DOI | 10.1007/978-1-4939-7395-8_15 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7394-1, 978-1-4939-7395-8
|
| Authors |
Maryam Agboluaje, Dominic Sauvageau, Agboluaje, Maryam, Sauvageau, Dominic |
| Abstract |
The optimal conditions for the production of virulent bacteriophages in bioreactors can vary greatly depending on the host-bacteriophage system used. We present a general method for the production of virulent bacteriophages in bioreactors that can be adapted to many host-bacteriophage systems and various operating conditions (reactor volume, medium composition, temperature, etc.). The procedures detail how to establish optimal initial infection conditions (infection load and initial multiplicity of infection (MOI)), prepare the host pre-culture and bioreactor, operate the bioreactor, and harvest the bacteriophage product. Batch operation is detailed but a short discussion addresses other modes of operation, namely two-stage continuous bioreactors and two-stage cycling bioreactors. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 50% |
| Scientists | 1 | 50% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 68 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 12 | 18% |
| Student > Bachelor | 12 | 18% |
| Researcher | 9 | 13% |
| Student > Ph. D. Student | 3 | 4% |
| Lecturer | 2 | 3% |
| Other | 6 | 9% |
| Unknown | 24 | 35% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 13 | 19% |
| Biochemistry, Genetics and Molecular Biology | 10 | 15% |
| Chemical Engineering | 5 | 7% |
| Immunology and Microbiology | 3 | 4% |
| Medicine and Dentistry | 2 | 3% |
| Other | 6 | 9% |
| Unknown | 29 | 43% |