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Photorespiration

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Cover of 'Photorespiration'

Table of Contents

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    Book Overview
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    Chapter 1 Estimation of Photorespiratory Fluxes by Gas Exchange
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    Chapter 2 Measurement of Transcripts Associated with Photorespiration and Related Redox Signaling
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    Chapter 3 Measurement of Enzyme Activities
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    Chapter 4 In Vitro Alkylation Methods for Assessing the Protein Redox State
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    Chapter 5 Dimethyl-Labeling-Based Quantification of the Lysine Acetylome and Proteome of Plants
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    Chapter 6 In Vitro Analysis of Metabolite Transport Proteins
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    Chapter 7 Quantification of Photorespiratory Intermediates by Mass Spectrometry-Based Approaches
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    Chapter 8 Targeted Isolation and Characterization of T-DNA Mutants Defective in Photorespiration
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    Chapter 9 Exploiting Natural Variation to Discover Candidate Genes Involved in Photosynthesis-Related Traits
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    Chapter 10 Metabolic Engineering of Photorespiration
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    Chapter 11 13CO2 Labeling and Mass Spectral Analysis of Photorespiration
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    Chapter 12 Isotopically Nonstationary Metabolic Flux Analysis (INST-MFA) of Photosynthesis and Photorespiration in Plants
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    Chapter 13 Genome-Scale Modeling of Photorespiratory Pathway Manipulation
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    Chapter 14 Kinetic Modeling of Photorespiration
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    Chapter 15 Investigating the Role of the Photorespiratory Pathway in Non-photosynthetic Tissues
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    Chapter 16 Studying the Function of the Phosphorylated Pathway of Serine Biosynthesis in Arabidopsis thaliana
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    Chapter 17 Light Microscopy, Transmission Electron Microscopy, and Immunohistochemistry Protocols for Studying Photorespiration
Attention for Chapter 3: Measurement of Enzyme Activities
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Citations

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Chapter title
Measurement of Enzyme Activities
Chapter number 3
Book title
Photorespiration
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-7225-8_3
Pubmed ID
Book ISBNs
978-1-4939-7224-1, 978-1-4939-7225-8
Authors

Hermann Bauwe

Abstract

The determination of enzyme activities in organ or organellar extracts is an important means of investigating metabolic networks and allows testing the success of enzyme-targeted genetic engineering. It also delivers information on intrinsic enzyme parameters such as kinetic properties or impact of effector molecules. This chapter provides protocols on how to assess activities of the enzymes of the core photorespiratory pathway, from 2-phosphoglycolate phosphatase to glycerate 3-kinase.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 24 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 24 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 5 21%
Researcher 3 13%
Student > Ph. D. Student 2 8%
Other 1 4%
Unknown 13 54%
Readers by discipline Count As %
Chemistry 3 13%
Agricultural and Biological Sciences 3 13%
Medicine and Dentistry 2 8%
Biochemistry, Genetics and Molecular Biology 1 4%
Engineering 1 4%
Other 0 0%
Unknown 14 58%