Chapter title |
Measurement of Transcripts Associated with Photorespiration and Related Redox Signaling
|
---|---|
Chapter number | 2 |
Book title |
Photorespiration
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-7225-8_2 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7224-1, 978-1-4939-7225-8
|
Authors |
Amna Mhamdi, Pavel I. Kerchev, Patrick Willems, Graham Noctor, Frank Van Breusegem |
Abstract |
To study photorespiration and to characterize related components, gene expression analysis is a central approach. An overview of the experimental setup, protocols, and methods we use to investigate photorespiration-associated gene expression is presented. Within this chapter, we describe simple procedures to experimentally alter the photorespiratory flux and provide protocols for transcriptomic analysis with a focus on genes encoding photorespiratory proteins as well as those induced by photorespiratory hydrogen peroxide (H2O2). Examples of typical results are presented and their significance to understanding redox signaling is discussed. |
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Mendeley readers
Geographical breakdown
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Unknown | 3 | 100% |
Demographic breakdown
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Professor | 1 | 33% |
Researcher | 1 | 33% |
Unknown | 1 | 33% |
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Agricultural and Biological Sciences | 2 | 67% |
Unknown | 1 | 33% |