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Mendeley readers
| Chapter title |
Quantification of Photorespiratory Intermediates by Mass Spectrometry-Based Approaches
|
|---|---|
| Chapter number | 7 |
| Book title |
Photorespiration
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-7225-8_7 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7224-1, 978-1-4939-7225-8
|
| Authors |
Stéphanie Arrivault, Toshihiro Obata |
| Abstract |
Photorespiration is an essential metabolic process in plants occurring via the oxygenase reaction of RuBisCO. In order to understand this process, it is essential to determine the amounts of intermediates involved. For this purpose we combined mass spectrometry-based approaches and the use of authentic standards for the quantification of photorespiratory intermediates. Here we describe protocols for the extraction and quantification of 2-phosphoglycolate (2PG) by LC-MS/MS and serine, glycine, glycolate, hydroxypyruvate, glyoxylate, and glycerate by GC-MS. |
Mendeley readers
The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 6 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Doctoral Student | 2 | 33% |
| Researcher | 1 | 17% |
| Unknown | 3 | 50% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 1 | 17% |
| Agricultural and Biological Sciences | 1 | 17% |
| Unknown | 4 | 67% |