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Mendeley readers
| Chapter title |
RAPD-PCR as a Rapid Approach for the Evaluation of Genotoxin-Induced Damage to Bacterial DNA
|
|---|---|
| Chapter number | 18 |
| Book title |
Fast Detection of DNA Damage
|
| Published in |
Methods in molecular biology, July 2017
|
| DOI | 10.1007/978-1-4939-7187-9_18 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7185-5, 978-1-4939-7187-9
|
| Authors |
Rosanna Tofalo, Aldo Corsetti |
| Abstract |
RAPD PCR is a sensitive and reliable approach useful for the detection of DNA lesions due to environmental contaminants. In addition, this method is cost-effective, and can be performed in any laboratory having a DNA thermocycler and gel electrophoresis system. Here, we describe its application to identify genotoxin-induced DNA damage in foodborne bacteria. DNA alterations are detected through the analysis of electrophoresis profiles with the appearance or disappearance of new bands as compared to the non-mutated control. The described RAPD PCR procedure takes 6 h for completion. It uses small amounts of DNA and can reveal even low mutation rates. |
Mendeley readers
The data shown below were compiled from readership statistics for 13 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 13 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 4 | 31% |
| Researcher | 3 | 23% |
| Student > Postgraduate | 2 | 15% |
| Student > Bachelor | 1 | 8% |
| Student > Ph. D. Student | 1 | 8% |
| Other | 0 | 0% |
| Unknown | 2 | 15% |
| Readers by discipline | Count | As % |
|---|---|---|
| Immunology and Microbiology | 3 | 23% |
| Agricultural and Biological Sciences | 2 | 15% |
| Biochemistry, Genetics and Molecular Biology | 2 | 15% |
| Medicine and Dentistry | 1 | 8% |
| Neuroscience | 1 | 8% |
| Other | 0 | 0% |
| Unknown | 4 | 31% |