Chapter title |
Rapid Detection of γ-H2AX by Flow Cytometry in Cultured Mammalian Cells
|
---|---|
Chapter number | 11 |
Book title |
Fast Detection of DNA Damage
|
Published in |
Methods in molecular biology, July 2017
|
DOI | 10.1007/978-1-4939-7187-9_11 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7185-5, 978-1-4939-7187-9
|
Authors |
Denis Firsanov, Liudmila Solovjeva, Olga Lublinskaya, Valeriy Zenin, Igor Kudryavtsev, Maria Serebryakova, Maria Svetlova |
Abstract |
Methods commonly used for detection of DNA double-strand breaks (DSBs) and analysis of cell death are generally time-consuming, and, therefore, any improvements in these techniques are important for researchers and clinicians. At present, flow cytometry is the most rapid method for detection of DSBs and cell viability. In this chapter, we provide our experience and methodological modification of flow cytometry protocol for the detection of γ-H2AX, a well-known marker of DSBs, in fixed mammalian fibroblasts. The modifications permit a reduction in the time required for DSB detection by flow cytometry. |
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