Chapter title |
Rapid Detection of Apoptosis in Cultured Mammalian Cells
|
---|---|
Chapter number | 8 |
Book title |
Methods in Molecular Biology
|
Published in |
Methods in molecular biology, July 2017
|
DOI | 10.1007/978-1-4939-7187-9_8 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7185-5, 978-1-4939-7187-9
|
Authors |
Kudryavtsev, Igor, Serebryakova, Maria, Solovjeva, Liudmila, Svetlova, Maria, Firsanov, Denis, Igor Kudryavtsev, Maria Serebryakova, Liudmila Solovjeva, Maria Svetlova, Denis Firsanov |
Abstract |
Flow cytometry is a powerful tool for the analysis of apoptosis, the process that directly determines cell fate after the action of different stresses. Here, we describe a flow cytometry method for the assessment of early and late stages of apoptosis in non-fixed cultured cells using SYTO16, DRAQ7, and PO-PRO1 dyes simultaneously. This multicolor flow cytometry procedure requires 45 min for completion and provides a quantitative assessment of cell viability. It can be useful in evaluating the cytotoxic properties of new drugs, and antitumor interventions. |
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