| Chapter title |
On Electrochemical Methods for Determination of Protein-Lipid Interaction
|
|---|---|
| Chapter number | 22 |
| Book title |
Lipidomics
|
| Published in |
Methods in molecular biology, June 2017
|
| DOI | 10.1007/978-1-4939-6996-8_22 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6995-1, 978-1-4939-6996-8
|
| Authors |
Zhiping Hu, Yanli Mao, Hu, Zhiping, Mao, Yanli |
| Editors |
Sanjoy K. Bhattacharya |
| Abstract |
Amyloid-β (Aβ) peptides are important and reliable molecular biomarkers for the diagnosis and prognosis of Alzheimer's disease. Aggregation and fibrillization of Aβ peptides on ganglioside GM1 (GM1)-containing lipid membranes is considered a cause of neurodegenerative disease. Because GM1 is abundant in the central nervous system and plays a key role in the aggregation of Aβ, the interaction of Aβ with supported planar lipid bilayers (SPBs) containing GM1 is of great significance. We have prepared SPBs containing GM1 in order to study the electrochemical characteristics of GM1/sphingomyelin/cholesterol SPBs and their interaction with Aβ(1-40) by cyclic voltammetry and electrochemical impedance spectroscopy (EIS), which proves that electrochemical is a promising method for analyzing the interaction between peptides and lipid membranes. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 7 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 4 | 57% |
| Other | 1 | 14% |
| Student > Master | 1 | 14% |
| Unknown | 1 | 14% |
| Readers by discipline | Count | As % |
|---|---|---|
| Neuroscience | 2 | 29% |
| Chemistry | 2 | 29% |
| Psychology | 1 | 14% |
| Decision Sciences | 1 | 14% |
| Unknown | 1 | 14% |