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Mendeley readers
Chapter title |
Purification and Validation of Lipid Transfer Proteins
|
---|---|
Chapter number | 19 |
Book title |
Lipidomics
|
Published in |
Methods in molecular biology, June 2017
|
DOI | 10.1007/978-1-4939-6996-8_19 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6995-1, 978-1-4939-6996-8
|
Authors |
Matti A. Kjellberg, Anders P. E. Backman, Anna Möuts, Peter Mattjus |
Editors |
Sanjoy K. Bhattacharya |
Abstract |
Understanding the holistic picture of lipid homeostasis not only involves the analysis of synthesis and breakdown of lipids but also requires a thorough understanding of their transport. The transport of lipid monomers in an aqueous environment is facilitated by different lipid transfer proteins. Their universal feature is the shielding or encapsulation of the hydrophobic part of the lipid, consequently overcoming the poor solubility of lipids in water. Here we describe a method to purify lipid transfer proteins using bacterial expression. We also present three methods to validate their transfer activity. |
Mendeley readers
The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 6 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 2 | 33% |
Student > Bachelor | 1 | 17% |
Unspecified | 1 | 17% |
Researcher | 1 | 17% |
Student > Postgraduate | 1 | 17% |
Other | 0 | 0% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 2 | 33% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 17% |
Unspecified | 1 | 17% |
Social Sciences | 1 | 17% |
Chemistry | 1 | 17% |
Other | 0 | 0% |