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Lipidomics

Overview of attention for book
Cover of 'Lipidomics'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Lipid Sample Preparation for Biomedical Research
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    Chapter 2 Lipid Extraction Techniques for Stable Isotope Analysis and Ecological Assays
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    Chapter 3 Isolation of Lipid Raft Proteins from CD133+ Cancer Stem Cells
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    Chapter 4 Isolation of Neuronal Synaptic Membranes by Sucrose Gradient Centrifugation
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    Chapter 5 Sample Preparation and Analysis for Imaging Mass Spectrometry
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    Chapter 6 Direct Measurement of Free and Esterified Cholesterol Mass in Differentiated Human Podocytes: A TLC and Enzymatic Assay-Based Method
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    Chapter 7 High-Performance Chromatographic Separation of Cerebrosides
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    Chapter 8 Lipid Identification by Untargeted Tandem Mass Spectrometry Coupled with Ultra-High-Pressure Liquid Chromatography
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    Chapter 9 Utility of Moderate and High-Resolution Mass Spectrometry for Class-Specific Lipid Identification and Quantification
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    Chapter 10 A Robust Lipidomics Workflow for Mammalian Cells, Plasma, and Tissue Using Liquid-Chromatography High-Resolution Tandem Mass Spectrometry
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    Chapter 11 Combined Use of MALDI-TOF Mass Spectrometry and 31P NMR Spectroscopy for Analysis of Phospholipids
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    Chapter 12 Global Monitoring of the Mammalian Lipidome by Quantitative Shotgun Lipidomics
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    Chapter 13 Bioinformatics Pertinent to Lipid Analysis in Biological Samples
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    Chapter 14 LC–MS-Based Lipidomics and Automated Identification of Lipids Using the LipidBlast In-Silico MS/MS Library
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    Chapter 15 Single-Step Capture and Targeted Metabolomics of Alkyl-Quinolones in Outer Membrane Vesicles of Pseudomonas aeruginosa
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    Chapter 16 Analysis of Fatty Acid and Cholesterol Content from Detergent-Resistant and Detergent-Free Membrane Microdomains
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    Chapter 17 Computational Functional Analysis of Lipid Metabolic Enzymes
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    Chapter 18 Isoprenylation of Monomeric GTPases in Human Trabecular Meshwork Cells
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    Chapter 19 Purification and Validation of Lipid Transfer Proteins
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    Chapter 20 Incorporation of Artificial Lipid-Anchored Proteins into Cultured Mammalian Cells
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    Chapter 21 Sonication-Based Basic Protocol for Liposome Synthesis
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    Chapter 22 On Electrochemical Methods for Determination of Protein-Lipid Interaction
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    Chapter 23 Angiogenesis Model of Cornea to Understand the Role of Sphingosine 1-Phosphate
Attention for Chapter 14: LC–MS-Based Lipidomics and Automated Identification of Lipids Using the LipidBlast In-Silico MS/MS Library
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Chapter title
LC–MS-Based Lipidomics and Automated Identification of Lipids Using the LipidBlast In-Silico MS/MS Library
Chapter number 14
Book title
Lipidomics
Published in
Methods in molecular biology, June 2017
DOI 10.1007/978-1-4939-6996-8_14
Pubmed ID
Book ISBNs
978-1-4939-6995-1, 978-1-4939-6996-8
Authors

Tomas Cajka, Oliver Fiehn, Cajka, Tomas, Fiehn, Oliver

Editors

Sanjoy K. Bhattacharya

Abstract

This protocol describes the analysis, specifically the identification, of blood plasma lipids. Plasma lipids are extracted using methyl tert-butyl ether (MTBE), methanol, and water followed by separation and data acquisition of isolated lipids using reversed-phase liquid chromatography coupled to quadrupole/time-of-flight mass spectrometry (RPLC-QTOFMS) operated in MS/MS mode. For lipid identification, acquired MS/MS spectra are converted to the mascot generic format (MGF) followed by library search using the in-silico MS/MS library LipidBlast. Using this approach, lipid classes, carbon-chain lengths, and degree of unsaturation of fatty-acid components are annotated.

X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 57 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Brazil 1 2%
Unknown 56 98%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 17 30%
Researcher 8 14%
Student > Bachelor 7 12%
Student > Doctoral Student 5 9%
Lecturer 4 7%
Other 8 14%
Unknown 8 14%
Readers by discipline Count As %
Chemistry 14 25%
Biochemistry, Genetics and Molecular Biology 13 23%
Agricultural and Biological Sciences 8 14%
Medicine and Dentistry 4 7%
Pharmacology, Toxicology and Pharmaceutical Science 3 5%
Other 2 4%
Unknown 13 23%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 27 March 2018.
All research outputs
#20,431,953
of 22,985,065 outputs
Outputs from Methods in molecular biology
#9,929
of 13,149 outputs
Outputs of similar age
#274,996
of 315,315 outputs
Outputs of similar age from Methods in molecular biology
#210
of 270 outputs
Altmetric has tracked 22,985,065 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,149 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 315,315 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 270 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.