Chapter title |
LC–MS-Based Lipidomics and Automated Identification of Lipids Using the LipidBlast In-Silico MS/MS Library
|
---|---|
Chapter number | 14 |
Book title |
Lipidomics
|
Published in |
Methods in molecular biology, June 2017
|
DOI | 10.1007/978-1-4939-6996-8_14 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6995-1, 978-1-4939-6996-8
|
Authors |
Tomas Cajka, Oliver Fiehn, Cajka, Tomas, Fiehn, Oliver |
Editors |
Sanjoy K. Bhattacharya |
Abstract |
This protocol describes the analysis, specifically the identification, of blood plasma lipids. Plasma lipids are extracted using methyl tert-butyl ether (MTBE), methanol, and water followed by separation and data acquisition of isolated lipids using reversed-phase liquid chromatography coupled to quadrupole/time-of-flight mass spectrometry (RPLC-QTOFMS) operated in MS/MS mode. For lipid identification, acquired MS/MS spectra are converted to the mascot generic format (MGF) followed by library search using the in-silico MS/MS library LipidBlast. Using this approach, lipid classes, carbon-chain lengths, and degree of unsaturation of fatty-acid components are annotated. |
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Demographic breakdown
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Members of the public | 1 | 100% |
Mendeley readers
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Brazil | 1 | 2% |
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Researcher | 8 | 14% |
Student > Bachelor | 7 | 12% |
Student > Doctoral Student | 5 | 9% |
Lecturer | 4 | 7% |
Other | 8 | 14% |
Unknown | 8 | 14% |
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Pharmacology, Toxicology and Pharmaceutical Science | 3 | 5% |
Other | 2 | 4% |
Unknown | 13 | 23% |