Chapter title |
Utility of Moderate and High-Resolution Mass Spectrometry for Class-Specific Lipid Identification and Quantification
|
---|---|
Chapter number | 9 |
Book title |
Lipidomics
|
Published in |
Methods in molecular biology, June 2017
|
DOI | 10.1007/978-1-4939-6996-8_9 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6995-1, 978-1-4939-6996-8
|
Authors |
Maria del Carmen Piqueras |
Editors |
Sanjoy K. Bhattacharya |
Abstract |
The study of lipidomics has been dramatically enhanced by using mass spectrometry techniques.With the purpose of identification and/or quantification of the lipids object of study, mass spectrometry has been proven to be the best approach, where three main techniques are currently being used: electrospray ionization (ESI), liquid chromatography (LC/MS), and matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (Han et al. Mass Spectrom Rev 31:134-178, 2012). In order to avoid the problems generated by gradient concentration using liquid chromatography, here we describe the method used for the lipidomics analysis using moderate and high-resolution mass spectrometry for class-specific lipid identification and quantification for phospholipid species using ESI-MS/MS. |
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