Chapter title |
Isoprenylation of Monomeric GTPases in Human Trabecular Meshwork Cells
|
---|---|
Chapter number | 18 |
Book title |
Lipidomics
|
Published in |
Methods in molecular biology, June 2017
|
DOI | 10.1007/978-1-4939-6996-8_18 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6995-1, 978-1-4939-6996-8
|
Authors |
Evan B. Stubbs Jr., Evan B. Stubbs |
Editors |
Sanjoy K. Bhattacharya |
Abstract |
Small monomeric GTPases, including those belonging to the Rho family, regulate a diverse array of intracellular signaling pathways which affect vesicle transport/trafficking, endocytosis, cell cycle progression, cell contractility, and formation of stress fibers or focal adhesions. Functional activation of newly synthesized small monomeric GTPases is facilitated by a multistep post-translational process involving transferase-catalyzed addition of farnesyl or geranylgeranyl isoprenoids to conserved cysteine residues within a unique carboxy terminal CaaX motif. Here, using well-established and widely available contemporary methodologies, detailed protocols by which to semi-quantitatively evaluate the functional consequence of post-translational isoprenylation in human trabecular meshwork cells are described. We introduce the concept that isoprenylation alone is itself a key regulator of mammalian Rho GTPase expression and turnover. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 5 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 1 | 20% |
Student > Bachelor | 1 | 20% |
Student > Doctoral Student | 1 | 20% |
Student > Master | 1 | 20% |
Unknown | 1 | 20% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 1 | 20% |
Sports and Recreations | 1 | 20% |
Chemistry | 1 | 20% |
Unknown | 2 | 40% |