| Chapter title |
Sample Preparation and Analysis for Imaging Mass Spectrometry
|
|---|---|
| Chapter number | 5 |
| Book title |
Lipidomics
|
| Published in |
Methods in molecular biology, June 2017
|
| DOI | 10.1007/978-1-4939-6996-8_5 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6995-1, 978-1-4939-6996-8
|
| Authors |
Genea Edwards, Annia Mesa, Robert I. Vazquez-Padron, Jane-Marie Kowalski, Sanjoy K. Bhattacharya |
| Editors |
Sanjoy K. Bhattacharya |
| Abstract |
Imaging mass spectrometry (IMS) is a novel quantitative technique used to investigative diverse biomolecules in tissue sections. Specifically, IMS uses analytical separation of mass spectrometry to determine the spatial distribution of certain lipids and/or proteins located directly on biological sections from a single tissue sample. Typically, IMS is combined with histological analysis to reveal additional distribution details of characterized biomolecules including cell type and/or subcellular localization. In this chapter, we describe the use of Matrix-Assisted Laser Desorption/Ionization (MALDI) Time-Of-Flight/Time-Of-Flight (TOF/TOF) to analyze various cholesterol and phosphatidylcholine species in atherosclerotic plaque of murine heart aortic valves. In particular, we detail animals used, tissue collection, preparation, matrix application, spectra acquisition for generating a color-coded image based on IMS spectral characteristics. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 2 | 40% |
| Canada | 1 | 20% |
| India | 1 | 20% |
| Unknown | 1 | 20% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 60% |
| Science communicators (journalists, bloggers, editors) | 1 | 20% |
| Scientists | 1 | 20% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 6 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 2 | 33% |
| Professor | 1 | 17% |
| Unknown | 3 | 50% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 2 | 33% |
| Biochemistry, Genetics and Molecular Biology | 1 | 17% |
| Unknown | 3 | 50% |