| Chapter title |
Global Monitoring of the Mammalian Lipidome by Quantitative Shotgun Lipidomics
|
|---|---|
| Chapter number | 12 |
| Book title |
Lipidomics
|
| Published in |
Methods in molecular biology, June 2017
|
| DOI | 10.1007/978-1-4939-6996-8_12 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6995-1, 978-1-4939-6996-8
|
| Authors |
Nielsen, Inger Ødum, Maeda, Kenji, Bilgin, Mesut, Inger Ødum Nielsen, Kenji Maeda, Mesut Bilgin |
| Editors |
Sanjoy K. Bhattacharya |
| Abstract |
The emerging field of lipidomics presents the systems biology approach to identify and quantify the full lipid repertoire of cells, tissues, and organisms. The importance of the lipidome is demonstrated by a number of biological studies on dysregulation of lipid metabolism in human diseases such as cancer, diabetes, and neurodegenerative diseases. Exploring changes and regulations in the huge networks of lipids and their metabolic pathways requires a lipidomics methodology: Advanced mass spectrometry that resolves the complexity of the lipidome. Here, we report a comprehensive protocol of quantitative shotgun lipidomics that enables identification and quantification of hundreds of molecular lipid species, covering a wide range of lipid classes, extracted from cultured mammalian cells. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 50% |
| Scientists | 1 | 50% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 20 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 4 | 20% |
| Student > Ph. D. Student | 3 | 15% |
| Researcher | 3 | 15% |
| Student > Master | 3 | 15% |
| Student > Doctoral Student | 1 | 5% |
| Other | 1 | 5% |
| Unknown | 5 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 5 | 25% |
| Agricultural and Biological Sciences | 3 | 15% |
| Chemistry | 3 | 15% |
| Nursing and Health Professions | 1 | 5% |
| Medicine and Dentistry | 1 | 5% |
| Other | 1 | 5% |
| Unknown | 6 | 30% |