Chapter title |
Zymography Detection of a Bacterial Extracellular Thermoalkaline Esterase/Lipase Activity
|
---|---|
Chapter number | 28 |
Book title |
Zymography
|
Published in |
Methods in molecular biology, June 2017
|
DOI | 10.1007/978-1-4939-7111-4_28 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7109-1, 978-1-4939-7111-4
|
Authors |
María Tapizquent, Marleny Fernández, Georgina Barreto, Zully Hernández, Lellys M. Contreras, Liliana Kurz, Jeff Wilkesman |
Editors |
Jeff Wilkesman, Liliana Kurz |
Abstract |
Lipases are esterases that occur widely in nature, yet those with commercial relevance are exclusively from microbial origin. Glycerol and long-chain fatty acids are the products after hydrolysis of esters bonds in saponifiable lipids catalyzed by lipases. In this work, we describe lipase/esterase activity contained in cell-free fractions from thermophilic bacteria, cultured in medium containing olive oil. Analysis of the cell-free fractions by electrotransference zymography, using tributyrin as substrate, revealed bands corresponding to lipase activity. The method is simple, fast, and inexpensive. |
Mendeley readers
Geographical breakdown
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Demographic breakdown
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Student > Bachelor | 2 | 20% |
Student > Master | 2 | 20% |
Researcher | 2 | 20% |
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Unspecified | 1 | 10% |
Other | 0 | 0% |
Unknown | 2 | 20% |
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Immunology and Microbiology | 1 | 10% |
Other | 0 | 0% |
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