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Mendeley readers
Chapter title |
A Sensitive, Rapid, and Specific Technique for the Detection of Collagenase Using Zymography
|
---|---|
Chapter number | 10 |
Book title |
Zymography
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Published in |
Methods in molecular biology, June 2017
|
DOI | 10.1007/978-1-4939-7111-4_10 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7109-1, 978-1-4939-7111-4
|
Authors |
Shivcharan Prasad, Ipsita Roy |
Editors |
Jeff Wilkesman, Liliana Kurz |
Abstract |
In-gel zymography is a commonly employed tool to identify active enzymes in a quantitative and qualitative manner. In this work, apart from the incorporation of substrate which is traditionally employed in zymography, the identification of collagenase by incubation of the enzyme resolved on a polyacrylamide gel with substrate solution is described. The two methods are quite fast and result in specific detection of bacterial collagenase. |
Mendeley readers
The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 7 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Professor | 2 | 29% |
Student > Bachelor | 2 | 29% |
Student > Doctoral Student | 1 | 14% |
Unknown | 2 | 29% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 2 | 29% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 14% |
Agricultural and Biological Sciences | 1 | 14% |
Chemistry | 1 | 14% |
Unknown | 2 | 29% |